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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Transl Res. 2021 May 15;237:1–15. doi: 10.1016/j.trsl.2021.05.002

Figure 7. A3 proteins interact with pVHL.

Figure 7.

8 million 293T cells were cultured in 10 cm plates for one day prior to transfection with tagged A3-proteins (10 μg) and Flag-tagged pVHL (10 μg). Complexes of pVHL with each A3 were affinity purified with anti-HA antibodies, except for A3A, which is Myc-tagged and was thusly purified with anti-Myc antibody. In addition, anti-Flag antibody was used to isolate A3-pVHL complexes in a complementary manner. (A) Immunoblot of the input and affinity purified tagged A3B and pVHL following pulldown with anti-HA and -Flag conjugated beads, respectively. (B) Immunoblots of expanded affinity pulldown experiments for additional members of the APOBEC3 family (A3A, -C, -F, -G, -H I, H II).