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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Transl Res. 2021 May 15;237:1–15. doi: 10.1016/j.trsl.2021.05.002

Figure 8. Addition of ARIH1 increases pVHL-mediated degradation of several A3s.

Figure 8.

293T cells were cultured in 6-well plates with 800,000 cells/well one day before co-transfection with an A3 expression plasmid (500 ng) and either an empty control vector, a plasmid expressing HA-tagged pVHL (500 ng), a plasmid expressing Flag-tagged ARIH1 (500 ng), or both the pVHL and ARIH1-expression plasmids (500 ng of each). In order to compare A3 protein levels upon expression of pVHL, ARIH1, or both, immunoblot experiments were performed. (A-H) Representative immunoblots of these experiments are shown. To evaluate if adding ARIH1 increases pVHL effect on each A3, the relevant comparison in each panel is the A3 band intensity in lane 2 with only pVHL over-expressed, relative to the A3 band intensity in lane 4 with both ARIH1 and pVHL over-expressed.