Figure 4. sHDL vaccine nanodiscs elicit strong antigen-specific CD8+ T cell responses.
A) C57BL/6 mice were immunized with nanodisc formulations (15.5 nmol Adpgk peptide, 2.3 nmol CpG, or 60 μg polyICLC) on days 0, 14, and 28. B) On days 7, 21, and 35, the frequency of Adpgk-specific CD8α+ T cells among PBMCs was measured. C) Shown are representative scatter plots for Adpgk-specific CD8α+ T cells on day 35. Data represent mean ± SEM, n = 5. Statistical significance was calculated by one-way ANOVA, followed by the Tukey’s multiple comparisons post-test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.