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. Author manuscript; available in PMC: 2022 Mar 8.
Published in final edited form as: Nat Cell Biol. 2021 Sep 8;23(9):978–991. doi: 10.1038/s41556-021-00732-8

Extended Data Fig. 2. Expression of TRIM15 in melanoma cells and its role in ERK activation.

Extended Data Fig. 2

a, Expression of TRIM15 and CYLD, and phosphorylation of ERK1/2 and ELK-1, in melanoma cell lines. b, Tumor cell lines used in the current work and the status of BRAF, NRAS, and KRAS mutations. WM3960 was a cell line established from patient-derived xenografts (PDX) tumors58. c, HEK293T cells transfected with Flag-ERK1 and/or HA-TRIM15 were analyzed for Flag-ERK1 phosphorylation by anti-Flag d-IP, followed by Western blot. d, e, ERK1/2 activation in HT29 cells (which harbors BRAFV600E) stably expressing control (shCtrl) or one of the two independent TRIM15 shRNAs (#1 and #2) (d), and in parental and TRIM15-knockout SK-MEL-173 cells (e). f, g, Levels of cyclin D1 and BCL2 proteins in A375 cells transfected with control (Ctrl) or TRIM15 siRNA (f), or treated with vehicle (DMSO) or trametinib (2 μM) for 24 h.