|
Bacterial Strains
|
|
E. coli, OP50 strain |
http://www.cgc.umn.edu
|
RRID: WB-STRAIN:OP50 |
|
Chemicals, Peptides, and Recombinant Proteins
|
| Levamisole |
http://www.acros.com
|
Cat # 187870100 |
| Agarose |
https://www.sigmaaldrich.com/
|
Cat # A9539 |
|
Experimental Models: Organisms/Strains
|
| Strain name |
Genotype |
Reference |
|
C. elegans Strain PT1435: pha-1(e2123) III; myIs1 IV; him-5(e1490) V; myEx552 [Ppkd-2::TBB-4::tdTomato]
|
[51] |
RRID: WB-STRAIN: PT1435 |
|
C. elegans Strain PT823: pha-1(e2123) III; myIs1 [PKD-2::GFP+Punc-122::GFP]IV; him-5(e1490)V; myEx431 [Pnphp-1::NPHP-1::dsRED] |
[51] |
RRID: WB-STRAIN: PT823 |
|
C. elegans Strain PT621: him-5(e1490) myIs4 [PKD-2::GFP+Punc-122::GFP]V
|
[52] |
RRID: WB-STRAIN: PT621 |
|
C. elegans Strain PT3112: pha-1(e2123) III; him-5(e1490) V myIs4V; myEx888[CIL-7::tagRFP + pBX1].
|
[25], this paper |
RRID: WB-STRAIN: PT3112 |
|
C. elegans Strain PT3383: klp-6(my8) III; him-5(e1490) myIs4V; myEx888[CIL-7::tagRFP + pBX1]
|
This paper |
RRID: WB-STRAIN: PT3383 |
|
C. elegans Strain PT3380: pha-1(e2123) III; osm-3(p802) klp-11(tm324)IV; him-5(e1490) myIs4V; myEx888[CIL-7::tagRFP + pBX1]
|
This paper |
RRID: WB-STRAIN: PT3380 |
|
C. elegans Strain PT2681: cil-7(tm5848) I; myIs1[PKD-2::GFP+Punc-122::GFP] IV; him-5(e1490)V
|
This paper |
RRID: WB-STRAIN: PT2681 |
|
C. elegans Strain PT3602: cil-7(my61[cil-7::mNG])I; him-5(e1490) V
|
This paper |
RRID: WB-STRAIN: PT3602 |
|
C. elegans Strain PT3621: cil-7(my61[cil-7::mNG])I; pkd-2(sy606)IV; him-5(e1490) V
|
This paper |
RRID: WB-STRAIN: PT3621 |
|
Software and Algorithms
|
| Metamorph |
http://www.moleculardevices.com
|
N/A |
| Zen Blue 2.0 |
https://www.zeiss.com
|
N/A |
| Zen Black 2.0 |
https://www.zeiss.com
|
N/A |
| Imaris 9.5 |
https://imaris.oxinst.com
|
N/A |
| Prism 8 |
http://www.graphpad.com
|
N/A |
| Python 3 |
Python Software Foundation |
https://www.python.org
|
| Code for calculating distance between centroids of EV particles and EV colocalization analysis |
This paper |
DOI: 10.5281/zenodo.4927186 |
|
Oligonucleotides
|
| 5’-ATGGATACGCTAACAACTTGGGCTGGGAGTCGATACATGGT-3’ |
This paper |
pJW3112F |
| 5’-CAGCTCTTCGCCCTTAGACACATGATGTGCAGACTTCTTCTTTC-3’ |
This paper |
pJW3112R |
| 5’ – CTCCATTGGAGCAACCTGTCAAATCTGGAAAGAAGAAGTCTGCACATCATGGAGGTGGCGGATCTGGAGGTGGAGGCTCTGGAGGAGGTGGATCTATGGTGTCGAAGGGAGAAG – 3’ |
This paper |
mNG forward homology donor Primer |
| 5’ – ATCACACAAAAATAGGCTGTTAAATGAAAATTATCGCAGTGGCATCTTATCGT AGCTACTTGTAGAGTTCATCCATTC – 3’ |
This paper |
mNG reverse homology donor Primer |
| 5’- TGACTACGATAAGATGCCAC – 3’ |
This paper |
crRNA target sequence |
|
Recombinant DNA
|
| Plasmid [CIL-7::tagRFP] |
This paper |
pJW3112 |
