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Microfluidics-based biomechanical assay with controlled oxygen tension environment. (A) A schematic of the experimental setup. (B) Timeline of the mechanical measurements of RBCs along the DeOxy-Oxy cycling. (C) The microfluidic chip for electro-deformation of single cells in suspension. (D) Exploded view drawing of the microfluidic chip. (E) Microscopic images of RBCs in free suspension when DEP field was OFF (left) and being trapped and elongated when DEP field was ON (right).
