Table 4.

Oligonucleotide primers and PCR conditions used in this study^†.

PCR	Primer name (sequence)	Annealing temperature (°C)	Product size (bp)	References
CTP synthase gene (pyrG) (external primer)	pyrG-1F (5′-ATTGCAAGTTCTGAGAATA-3′)	45	801	20
	pyrG -1R (5′-CAAACATTACGAGCAAATTC-3′)
CTP synthase gene (pyrG) (internal primer)	pyrG-2F (5-′GATATGGAAAATATTTTATTTATTG-3′)	49	707	20
	pyrG-2R (5′-AAACCAAGACAAATTCCAAG-3′)
	Borrel-ospAF1 (5′-GGGAATAGGTCTAATATTAGC-3′)	52	427	17
Outer surface protein A (ospA) (external primer)	Borrel-ospAR1 (5′-CTGTGTATTCAAGTCTGGTTCC-3′)
Outer surface protein A (ospA) (internal primer)	Borrel-ospAF2 (5′-CAAAATGTTAGTAGCCTTGAT-3′)	52	314	17
	Borrel-ospAR2 (5′-TCTGTTGATGACTTGTCTTT-3′)

^†bp, base pair; PCR, polymerase chain reaction.