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. 2021 Sep 14;11:18270. doi: 10.1038/s41598-021-97287-3

Figure 2.

Figure 2

Construction of the ku70 gene-deficient mutant in the T. asahii MPU129 strain by the ATMT system. (a) Structure of the plasmid used to construct the ku70 gene-deficient mutant and the predicted genome of the ku70 gene-deficient mutant. (b) The wild-type (MPU129) and ku70 gene-deficient candidate were spread on Sabouraud agar medium with or without G418 (50 µg/ml) and incubated at 27 °C for 2 days. (c) Location of the primers for confirming the genome structure of the ku70 gene-deficient candidate by PCR. (d) Confirmation of the ku70 gene-deficiency of the ku70 gene-deficient candidate by PCR using extracted genome DNA. Cropped blots were used. Full-length blots are presented in Supplementary Fig. 1.