Table 2.

Primers used in this study.

Primers	Nucleic acid sequence
pAg1-ku70(3′UTR)-nptII-ku70(5′UTR) for cloning
F ku70(5′UTR)	GCGGTACTAGTCGCCACCACGGTAGCGGTA
R ku70(5′UTR)	ACAAGATCTTGACGTCCTTTGGATGTTGCT
F nptII	ATGATTGAACAAGATGGATTGC
R nptII	TCAGAAGAACTCGTCAAGAAG
F ku70(3′UTR)	GCGGATCCAGTGTACTAGCGTGACGCTAGA
R ku70(3′UTR)	CTGGCGGTACCGCTGGCCGACCCACTCGTA
pAg1-cnb1(3′UTR)-NAT1-cnb1(5′UTR) for cloning
F cnb1(5′UTR)	TGAACTAGTCCGTGATCTGCTGCACGTTCGGGTCC
R cnb1(5′UTR)	AAAGGGCCCAAGATCTAGTGATAGATGTGTGGAGA
F cnb1(3′UTR)	CTGGGATCCGCGCGCACACACGGATGTGAGCGTAA
R cnb1(3′UTR)	CGCGGTACCACTGTTCACCTCTGGCATTGTTACGA
Genotyping
Primers-1 for ku70 genotyping
F ku70 gene locus	TCGAGGTCGCGACTTTGTTATTGCCAGGTCCTGA
R ku70 gene locus	AGAGCTGCGATCGTGGGCTGATCCGTCC
Primers-2 for ku70 genotyping
F ku70 gene ORF	TTTCAGCAACTCCGTCAGATCAGCGCCGAAGACA
R ku70 gene ORF	ATCTGCGAAAGAGCGGCCGGGCC
Primers-3 for ku70 genotyping
F ku70 gene outside	TTCATCGACTGTGGCCGGCTGGGTGTGG
R ku70 gene outside	GGACGAGATGGCCGGGGACCGGCTC
Primers-1 for cnb1 genotyping
F cnb1 gene locus	GGAGTGAAGAAGGGCAGAGAGCAACAACAGCGGT
R cnb1 gene locus	CCGTGATCGCATGGGGCGTGCACAAAGTG
Primers-2 for cnb1 genotyping
F cnb1 gene ORF	CGGCTCGGGTACGGTAGACTTCCAGGAGTTTGTCG
R cnb1 gene ORF	AACAGGTCCTCGAGCGTCATCTGCTTGACGATGT
Primers-3forcnb1genotyping
F cnb1 gene outside	GGACGGCGAGCAGGCGCTCTACATGAGC
R cnb1 gene outside	CTGAGTCCCATCGGCCCTTGCCTTCAAGCTACC
Amplification of cnb1 cassette for electroporation
F cnb1-cassette	CCGTGATCTGCTGCACGTTCGGGTCCG
R cnb1-cassette	CTGTTCACCTCTGGCTACGACCCCCTCCTC