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. 2021 Sep 14;11:18258. doi: 10.1038/s41598-021-97764-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of the balancer sC4 and a new complex rearrangement in BC4586 on LGIV. (A) Coverage analysis of the region surrounding the deletion of the segment V:16,060,619–19,331,432 in BC4586, revealing a large deletion. (B) PCR gels validating SVs and complex rearrangements in BC4586. 1 = Direct tandem duplication III:5,059,444–5,063,035; 2 = Deletion IV:9,853,074–9,853,123, inversion IV:9,853,123–9,853,675 and deletion IV:9,853,675–9,857,585; 3 = Direct tandem duplication IV:9,857,585–9,862,397; 4 = Deletion sC4 V:16,060,619–19,331,432; 5 = Deletion V:20,780,774–20,781,638. The breakpoints validated with the PCR no2 and no3 are part of the same chromosomal rearrangement. (C) Representative karyotypes for N2 and BC4586, confirming sC4 chromosome fusion (IV;V) (indicated by a white arrow) in BC4586. The scale bar represents 4 uM. (D) Schematic representation of the chromosomal rearrangement on LGIV. The successive SVs are displayed along the genomic region IV:9,851,500–9,863,000. The chromosomal rearrangement is composed of two deletions (blue), one direct tandem duplication (red), and one inversion (green). IGV screenshot of BC4586 aligned reads along the genomic region IV:9,851,500–9,863,000.