Skip to main content
. 2021 Sep 14;12:5446. doi: 10.1038/s41467-021-25758-2

Fig. 2. EOMES and T-BET promote NK cell survival and differentiation at different cellular transitions.

Fig. 2

a Flow cytometry analysis of NK cell percentage in BM or spleen lymphocytes of the indicated mice. Each dot corresponds to a single mouse (n = 5–6 mice in each group, pooled from 2 experiments). b Number of NK cells calculated from data in (a) combined with numeration of corresponding organs (n = 5–6, pooled from 2 experiments). c Proportion and numbers of NK cell subsets in BM or spleen lymphocytes of the indicated mice. Each dot corresponds to a single mouse (n = 5–6 mice in each group, pooled from 2 experiments). d NK cells of the indicated subsets (CD11B- or DP) and genotypes were FACS-sorted and then adoptively transferred into congenic unirradiated Ly5a X B6 mice (CD45.1/2). Two weeks later, spleen NK cells were purified from these mice and the CD11B/CD27 phenotype of transferred NK cells (identified by their CD45.2 expression) was analyzed by flow cytometry (n = 2–6, pooled from 2 experiments). In all graphs in Fig. 2, the mean measurement ± SD is shown. Unpaired t tests (two-tailed) were used for statistical analysis of data presented in this figure.