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. 2021 Sep 14;12:5446. doi: 10.1038/s41467-021-25758-2

Fig. 4. EOMES and T-BET have mostly distinct roles on NK cell transcription during maturation.

Fig. 4

RNA-seq analysis of sorted Tbx21−/−, Eomes−/− and appropriate control CD11B- and CD27− NK cells (n = 3 per group with three sorts in total). DEG were selected based on adjusted p < 0.05 (as determined by use of negative binomial generalized linear models). a Volcano plots of gene expression in immature CD11B- or mature CD27− NK cells comparing T-BET or EOMES deficient cells with appropriate controls. Selected genes are highlighted with their names. b Bar histograms showing the number of genes induced or repressed by T-BET or EOMES in immature and mature NK cells. c Venn diagram showing the overlap between the Immgen-defined NK cell signature and the EOMES-activated and T-BET-activated genes in immature and mature NK cells. d Venn diagram showing the overlap between T-BET regulated and EOMES-regulated genes in immature and mature NK cells. e Volcano plots comparing the expression of T-BET or EOMES dependent genes between WT immature and mature NK cells. Selected genes are highlighted with their names. DEG were selected based on adjusted p < 0.05 (as determined by use of negative binomial generalized linear models). f Venn diagram showing the overlap between T-BET-dependent, EOMES-dependent and maturation-regulated genes.