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. 2021 Sep 14;11:18238. doi: 10.1038/s41598-021-97501-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of Fc content of IgG by Western blot analysis. Immunoblot analysis of the SL PAV B1, B2 and purified horse IgG was done by using anti horse IgG HRP conjugated Fc region-specific antibody. M represents protein molecular mass marker and B1 and B2 represent batch1 and batch 2 of PAV, respectively. The primary antibody for both the analyses was raised against Fc region of IgG.