Table 2.
Determination of lethality of SL venoms (LD50 value) and neutralization of lethality (ED50 value) of venom samples by two batches of SL PAV.
| SL snake venom | LD50 value (µg/mouse) | ED50 value (mg/mL) | ED50 value (µL antivenom required/mg venom) | ||
|---|---|---|---|---|---|
| SL PAV B1 | SL PAV B2 | SL PAV B1 | SL PAV B2 | ||
| Naja naja | 20.33 ± 1.16 | 0.732 ± 0.04 | 0.687 ± 0.06 | 1400 ± 0.08 | 1500 ± 0.13 |
| Bungarus caeruleus | 1.50 ± 0.25 | 0.523 ± 0.03 | 0.556 ± 0.06 | 1900 ± 0.09 | 1800 ± 0.18 |
| Daboia russelii | 8.75 ± 0.30 | 0.786 ± 0.02 | 0.710 ± 0.04 | 1300 ± 0.04 | 1400 ± 0.08 |
| Echis carinatus | 10.85 ± 0.21 | 0.586 ± 0.03 | 0.586 ± 0.02 | 1700 ± 0.09 | 1700 ± 0.07 |
| Hypnale hypnale | 30.19 ± 1.90 | 0.725 ± 0.06 | 0.689 ± 0.05 | 1400 ± 0.12 | 1500 ± 0.11 |
Neutralization activity by PAV is expressed as mg venom neutralized per mL of antivenom. The venom stock (1 mg/mL) was used and injected in the mice at different dilution (1:8, 1:10, 1:12.5). The LD50 and ED50 values given below are means ± SD of five values.