Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 14;12:5435. doi: 10.1038/s41467-021-25742-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Average current density of HEK293 cells in response to a decrease of the intracellular ionic strength. Currents from WT cells (endog.) with a pipette solution containing 125 mM salt (black, n = 11), additional 1 µM of Sb1 (red, n = 10) or from cells expressing Sb1 (magenta, n = 12). b Currents of cells expressing Sb1 at indicated intracellular ion concentrations (125 mM Cl_i⁻, n = 12; 100 mM Cl_i⁻, n = 6; 75 mM Cl_i−, n = 18). Non-transfected cells (endog., n = 13) and cells expressing Sbn (n = 11), both recorded at 125 mM Cl_i⁻, are shown for comparison. c Currents (at 125 mM Cl_i⁻) from cells expressing different inhibitory sybodies (endog, n = 13; Sb1, n = 12; Sb2, n = 8; Sb3, n = 8). d Average response (at 175 mM Cl_i⁻) of HEK293 cells expressing the potentiating sybodies Sb4 (blue, n = 17) and Sb5 (cyan, n = 21) compared to WT (black, n = 20). e Current-densities of cells expressing Sb4 and Sb5 in comparison to WT cells at 175 mM (endog., n = 20; Sb4, n = 17; Sb5, n = 21) and 125 mM Cl_i⁻(endog., n = 13; Sb4, n = 7; Sb5, n = 9). f Average response of LRRC8^−/−cells expressing mouse LRRC8A (black, n = 11) or LRRC8A and the respective sybodies Sb4 (blue, n = 10), Sb5 (cyan, n = 11) and Sb3 (red, n = 11) at 75 mM Cl_i⁻. g Mean current-densities of LRRC8^−/−cells expressing mouse LRRC8A (black) and the indicated sybodies (LRRC8A, n = 11; Sb4, n = 10; Sb5, n = 11; Sb3, n = 11). Currents are recorded at 100 mV (a, d, f, g) or 80 mV (b, c, e) by patch-clamp electrophysiology in the whole-cell configuration. b, c, e, g Values of individual measurements are displayed as circles, mean current levels as bars. Asterisks indicate significant deviations from WT (endog.) in a two-sided one sample t-test, ns refers to non-significant differences. (b Sbn p = 0.96, Sb1 125 mM p < 0.0001, Sb1 100 mM p = 0.0004, Sb1 75 mM p < 0.0001, c Sb2 p < 0.0001, Sb3 p = 0.0006, d Sb4 175 mM p < 0.0001, Sb5 175 mM p = 0.0023, Sb4 125 mM p = 0.147, Sb5 125 mM p = 0.087, g Sb4 p = 0.038, Sb5 p = 0.0006, Sb3 p = 0.0009). a–g Data are from independent biological replicates, errors are s.e.m.