Table 2.
Cryo-EM data collection, refinement, and validation statistics.
| Dataset 1 LRRC8A/Sb1 (EMD-13202) (PDB 7P5V) | Dataset 2 LRRC8A/Sb2 (EMD-13203) (PDB 7P5W) | Dataset 3 LRRC8A/Sb3 (EMD-13208) (PDB 7P5Y) | Dataset 4 LRRC8A/Sb4 (EMD-13212) | Dataset 5 LRRC8A/Sb40.5 (EMD-13213) (PDB 7P60) | Dataset 6 LRRC8A/Sb5 (EMD-13230) (PDB 7P6K) | |
|---|---|---|---|---|---|---|
| Data collection and processing | ||||||
| Microscope | FEI Titan Krios | FEI Titan Krios | FEI Titan Krios | FEI Titan Krios | FEI Titan Krios | FEI Titan Krios |
| Camera | Gatan K3 GIF | Gatan K3 GIF | Gatan K3 GIF | Gatan K3 GIF | Gatan K3 GIF | Gatan K3 GIF |
| Magnification | 130,000 | 130,000 | 130,000 | 130,000 | 130,000 | 130,000 |
| Voltage (kV) | 300 | 300 | 300 | 300 | 300 | 300 |
| Electron exposure (e–/Å2) | 61 | 61 | 61 | 61 | 61 | 61 |
| Defocus range (μm) | −2.4 to −1.0 | −2.4 to −1.0 | −2.4 to −1.0 | −2.4 to −1.0 | −2.4 to −1.0 | −2.4 to −1.0 |
| Pixel size (Å)* | 0.651 (0.326) | 0.651 (0.326) | 0.651 (0.326) | 0.651 (0.326) | 0.651 (0.326) | 0.651 (0.326) |
| Initial number of micrographs (no.) | 5494 | 5633 | 6475 | 6416 | 4869 | 5199 |
| Initial particle images (no.) | 579,709 | 330,072 | 756,017 | 313,757 | 507,983 | 530,412 |
| Final particle images (no.) | 59,962 | 65,959 | 76,350 | 14,718 | 38,121 | 48,917 |
| Symmetry imposed | C3 | C3 | C3 | C3 | C3 | C3 |
| Map resolution FL, TM (Å) | ||||||
| FSC threshold 0.143 | 3.1, 2.7 | 3.5, 3.0 | 3.3, 2.9 | 7.7, - | 3.8, 3.5 | 3.8, 3.5 |
| Map resolution range (Å) | 2.6–6 | 2.8–12 | 2.9–6 | 7–10 | 3.1–8 | 3.3–8 |
| Refinement | ||||||
| Model resolution (Å) | ||||||
| FSC threshold 0.5 | 3.26 | 3.8 | 3.5 | - | 3.9 | 5.7 |
| Map sharpening b-factor (Å2) | −36 | −89 | −69 | −76 | −66 | |
| Model composition | ||||||
| Non-hydrogen atoms | 41,280 | 41,256 | 41,106 | 38,409 | 38,109 | |
| Protein residues | 5034 | 5028 | 5028 | 4680 | 4650 | |
| B factors (Å2) | ||||||
| Protein | 47.0 | 138.1 | 34.9 | 138.6 | 76.8 | |
| R.m.s. deviations | ||||||
| Bond lengths (Å) | 0.004 | 0.002 | 0.002 | 0.004 | 0.002 | |
| Bond angles (°) | 0.514 | 0.487 | 0.491 | 0.552 | 0.469 | |
| Validation | ||||||
| MolProbity score | 2.1 | 2.3 | 2.1 | 2.5 | 2.2 | |
| Clashscore | 9.0 | 10.7 | 10.3 | 14.6 | 9.5 | |
| Poor rotamers (%) | 3.5 | 3.7 | 2.6 | 5.5 | 3.9 | |
| Ramachandran plot | ||||||
| Favored (%) | 96.6 | 95.6 | 96.5 | 96.0 | 96.4 | |
| Allowed (%) | 3.4 | 4.4 | 3.5 | 4.0 | 3.6 | |
| Disallowed (%) | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
*Values in parentheses indicate the pixel size in super-resolution.