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. 2021 Jan 25;29(9):1396–1404. doi: 10.1038/s41431-020-00807-4

Fig. 3. Mimicking the oocyte death phenotype in mouse oocytes in vitro.

Fig. 3

A Images of mouse pb1 oocytes before and after fertilization. GV oocytes injected with WT (600 or 1000 ng/μl), p.(Ser238Pro) (600 or 1000 ng/μl), p.(Arg300Gln) (600 or 1000 ng/μl) or p.(Cys347Ser) (200 or 400 ng/μl) PANX1 cRNAs were allowed to mature in vitro for 10 h and before using for IVF. Arrows indicate dead oocytes. Scale bars, 100 μm. B, C The percentage of oocytes that died after fertilization at different concentrations of cRNAs injection. Three independent experiments were performed, each group includes 60–80 oocytes, and the data are shown as the means ± SEM.