FIG. 1.
CIF150 (hTAFII150) seems to be less tightly associated with holo TFIID than hTAFII250 and hTAFII130. (A) Recombinant and native CIF150 have an electrophoretic mobility in SDS–6% PAGE very similar to that of hTAFII130. Shown is a side-by-side immunoblot analysis of recombinant CIF150 (Rec.) (lane 1; 3 μl), native CIF150 (lane 2; 10 μl of HeLA nuclear extract [NE]) in comparison to hTAFII130 (lane 4; 10 μl of HeLA nuclear extract [NE]). Lane 3 contains molecular mass (M) standards (in kilodaltons). (B) Gel filtration experiments with HeLa nuclear extracts (buffer A [10% glycerol, 0.1 M KCl]) indicate a dissociation of CIF150 and human TFIID. Immunoblot assays were performed with 4 μl of HeLa nuclear extract (lane 1) (L) and gel filtration fractions 1 to 17 (lane 2 to 18, 20 μl loaded). The elutions of molecular mass standards are indicated. V0, voided volume.