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. 2021 Sep 14;12:5449. doi: 10.1038/s41467-021-25713-1

Fig. 2. Schematic overview of enzymatic modification of erythrocytes followed by glycomic analysis of N-glycosylation and their use in hemagglutination assays.

Fig. 2

a Neuraminidase A.U.: Neuraminidase from Arthrobacter ureafaciens; B3GnT2: β-1,3-N-acetylglucosaminyltransferase 2; B4GalT1: β-1,4-galactosyltransferase 1; ST6Gal1: α-2,6-sialyltransferase 1. b The 30 most abundant N-glycans on the enzymatically modified erythrocytes from chicken and turkey (based on relative intensity, excluding high-mannose type N-glycans, for all structures refer to Data S2) sorted by abundance and number of LacNAc units. Proposed structures are assigned to detected glycan compositions. c A/NL/816/91, A/NL/109/03, A/NL/761/09, A/NL/1797/17, A/NL/371/19, A/NL/622/12, A/NL/10006/19, and A/NL/384/19 tested with modified erythrocytes (2,6-Sia Poly-LN) from chicken (blue) and turkey (red). Unmodified, 2,6 resialylated (2,6-Sia) and extended desialylated (Poly-LN) erythrocytes were added as controls. Assays were performed in biological triplicates in the presence of oseltamivir and the means ± SEM were plotte.