Table 1.
Hemagglutination assay of A/H3N2 virus isolates using unmodified and 2,6-Sia Poly-LacNAc fowl erythrocytes and unmodified guinea pig erythrocytes.
| Virus | Type | Clade | Unmodified | 2,6-Sia | 2,6-Sia Poly-LN | Unmodified | 2,6-Sia | 2,6-Sia Poly-LN | Unmodified |
|---|---|---|---|---|---|---|---|---|---|
| Chicken | Turkey | Guinea pig | |||||||
| Hemagglutination titers (HAU per 25 μl) | |||||||||
| A/Bilthoven/16190/68 | H3N2 | Unknown | 24 | 32 | 64 | 64 | 48 | 64 | 48 |
| A/Beijing/353/89 | H3N2 | Unknown | 24 | 64 | 64 | 64 | 64 | 96 | 64 |
| A/Netherlands/816/91 | H3N2 | Unknown | 64 | 128 | 128 | 64 | 128 | 128 | n.t. |
| A/Netherlands/109/03 | H3N2 | Unknown | Neg. | 256 | 256 | 64 | 128 | 128 | n.t. |
| X-161B (A/Wisconsin/067/05) | H3N2 | Unknown | 12 | Neg. | 64 | 32 | 32 | 64 | n.t. |
| IVR-147 (A/Brisbane/10/07) | H3N2 | Unknown | 4 | Neg. | 8 | 16 | Neg. | 8 | n.t. |
| A/Netherlands/761/09 | H3N2 | Unknown | Neg. | Neg. | 64 | Neg. | 64 | 64 | n.t. |
| X-223A (A/Texas/50/12) | H3N2 | 3C.1 | 8 | Neg. | 8 | 12 | Neg. | 8 | n.t. |
| NIB-104 (A/Singapore/INFH-16-0019/16) | H3N2 | 3C.2a1 | 8 | 2 | 16 | 24 | 12 | 24 | 32 |
| A/Netherlands/2413/16 | H3N2 | 3C.2a1 | Neg. | Neg. | 96 | 3 | 24 | 128 | n.t. |
| A/Netherlands/751/17 | H3N2 | 3C.2a1 | Neg. | Neg. | 6 | Neg. | Neg. | 512 | 1 |
| A/Netherlands/757/17 | H3N2 | 3C.2a1 | Neg. | Neg. | 64 | 6 | 16 | 128 | n.t. |
| A/Netherlands/1797/17 | H3N2 | 3C.2a1 | Neg. | Neg. | 32 | Neg. | Neg. | 32 | Neg. |
| A/Netherlands/295/19 | H3N2 | 3C.2a1b | Neg. | Neg. | 192 | Neg. | Neg. | 192 | n.t. |
| A/Netherlands/314/19 | H3N2 | 3C.2a1b | Neg. | Neg. | 128 | Neg. | Neg. | 192 | Neg. |
| A/Netherlands/371/19 | H3N2 | 3C.2a1b | Neg. | Neg. | 32 | Neg. | Neg. | 32 | Neg. |
| A/Netherlands/03466/17 | H3N2 | 3C.2a | Neg. | Neg. | 2048 | Neg. | 6 | 512 | Neg. |
| NIB-112 (A/Switzerland/8060/17) | H3N2 | 3C.2a2 | n.t. | n.t. | n.t. | Neg. | Neg. | 4 | Neg. |
| A/Netherlands/10616/18 | H3N2 | 3C.2a2 | Neg. | Neg. | 3 | Neg. | Neg. | 4 | Neg. |
| A/Netherlands/622/12 | H3N2 | 3C.3 | Neg. | Neg. | 12 | Neg. | Neg. | 12 | 1 |
| A/Switzerland/9715293/13 | H3N2 | 3C.3a | Neg. | Neg. | 16 | 2 | 3 | 24 | 2 |
| A/Netherlands/153/16 | H3N2 | 3C.3a | Neg. | Neg. | 96 | 12 | 24 | 128 | n.t. |
| X-327 (A/Kansas/014/17) | H3N2 | 3C.3a | Neg. | 2 | 16 | 6 | 4 | 16 | 8 |
| A/Netherlands/384/19 | H3N2 | 3C.3a | Neg. | Neg. | 2048 | 12 | 192 | 1536 | 6 |
| A/Netherlands/10002/19 | H3N2 | 3C.3a | Neg. | Neg. | 64 | 12 | 16 | 96 | n.t. |
| A/Netherlands/10006/19 | H3N2 | 3C.3a | Neg. | 2 | 32 | 8 | 12 | 64 | 12 |
| IVR-180 (A/Singapore/GP1908/15) | H1N1 | 6B | 32 | 16 | 48 | 24 | 32 | 24 | 32 |
| BX-69A (B/Maryland/15/15) | B | n.a. | 32 | 16 | 64 | 32 | 32 | 32 | 32 |
Unknown clade designation was not used at the time of identification, n.t. not tested, n.a. not applicable, neg. no titer obtained.