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. 2021 Sep 1;12:721453. doi: 10.3389/fimmu.2021.721453

Figure 2.

Figure 2

Effects of IL-17 in salivary gland cells. (A) Hierarchical clustering shows gene expression in isolated salivary gland cells from NOD/ShiLtJ mice with or without T1D. Yellow and blue regions in cells indicate high and low relative expression levels, respectively. (B) Bar graphs show expression levels of indicated genes involved in the IL-17 receptor signaling pathway according to microarray analysis. (C) Human salivary gland cells were cultured with TNF-α in the absence or presence of recombinant human IL-17 or z-VAD for 48 h, and the expression levels of p-MLKL (left), RIPK3 (right), and β-actin were examined by Western blotting. Bar graphs show average expression levels of p-MLKL (left) and RIPK3 (right). (D) Micrographs show mouse salisphere sizes in cultured salivary gland stem cells (SGSCs) with IL-17. Original magnifications were 200× (top) and 40× (bottom). Bar graphs show average numbers of salispheres (left) and average secretion of α-amylase (right) under the indicated conditions. (E) Bar graphs show average transcription levels of AQP5, Amy1, Krt18, and Nanog under the indicated conditions. Values are means ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.