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Distance independence of TPA-induced HS2 enhancer activity. Cultures were treated with 5 nM TPA (shaded) or vehicle (unshaded) for 20 h before each analysis. Histograms show representative flow cytometric analyses of stably transfected K562 clonal cell lines containing HS2(2.2)γEGFP or HS2(7.5)γEGFP constructs. The number of integrated copies of the reporter plasmid is shown. Nonexpressing cells had a relative fluorescence of no more than 1 on this scale.
