TABLE 1.
Influence of TPA treatment on EGFP expression in stably transfected K562 clonal lines
| Clone | Copy no. | % EGFP-positive cells
|
Mean fluorescence
|
Induced/uninduced ratio | ||
|---|---|---|---|---|---|---|
| With TPA | Without TPA | With TPA | Without TPA | |||
| γEGFP-1 | 1 | 71.1 | 73.5 | 7.7 | 8.3 | 0.9 |
| γEGFP-4 | 8 | 99.9 | 99.8 | 492 | 531 | 0.9 |
| γEGFP-5 | 1 | 2.4 | 2.3 | 1.5 | 4.2 | 0.4 |
| HS2γEGFP-1 | 14 | 99.7 | 99.8 | 778 | 344 | 2.3 |
| HS2γEGFP-3 | 3 | 99.9 | 99.9 | 34 | 27 | 1.2 |
| HS2γEGFP-4 | 5 | 99.9 | 99.9 | 125 | 106 | 1.2 |
| HS2γEGFP-7 | 11 | 99.9 | 99.9 | 506 | 270 | 1.9 |
| HS2γEGFP-8 | 3 | 99.9 | 99.8 | 66 | 52 | 1.3 |
| HS2γEGFP-9 | 16 | 99.9 | 99.8 | 371 | 256 | 1.4 |
| HS2γEGFP-10 | 14 | 99.8 | 99.7 | 390 | 335 | 1.2 |
| HS3γEGFP-2 | 3 | 98.9 | 98.9 | 329 | 319 | 1.0 |
| HS3γEGFP-3 | 3 | 99.9 | 99.9 | 561 | 663 | 0.8 |
| HS3γEGFP-5 | 4 | 99.8 | 99.9 | 1,973 | 2,306 | 0.9 |
| HS3γEGFP-6 | 2 | 99.8 | 99.9 | 464 | 512 | 0.9 |
| HS3γEGFP-7 | 2 | 99.8 | 99.9 | 903 | 925 | 1.0 |
| HS3γEGFP-8 | 2 | 97.4 | 97.8 | 80 | 88 | 0.9 |