Figure 2.

Survival of broiler embryos after challenge with Salmonella Enteritidis strain SE_TAU19. An avian pathogenic E. coli strain known to be virulent to broiler embryos, EC_06YS, was used to challenge 21 eggs and served as a positive control. Eight broiler embryos were challenged with SE_TAU19. Eggs were inoculated with approximately 200 CFU/egg via the intra-allantoic route at 12 days of incubation and candled every 12 h to monitor survivability. All embryos challenged with SE_TAU19 were dead by 84 h, while the six PBS-injected control eggs survived and EC_06YS achieved expected lethality.