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. 2021 Sep 1;15:714611. doi: 10.3389/fncir.2021.714611

TABLE 4.

Morphological criteria for identifying VENs (adapted and modified from Banovac et al., 2019).

Morphological feature Characteristics of VENs Recommended staining method for optimal assessment
Apical process Very thick origin (up to 8 μm), very gradual decrease in thickness Rapid Golgi, Golgi-Cox, or other Golgi modifications that result in quality impregnation of the dendritic tree (possible alternative: intracellular dye injections)
Basal tree bifurcation degree Low
Basal process thickness Constant or very gradual decrease
Basal process arborization High, brush-like (basilar skirt)
Axon origin End of basal stem Rapid Golgi or other Golgi modifications that clearly visualize axon origin (possible alternative: intracellular dye injections)
Cell body shape Long (50–120 μm) and stick-shaped (5–10 μm wide), preferably with clear regional and laminar specificity Nissl, Golgi, or intracellular dye injections (possible alternatives: NeuN, SMI-32)