FIG. 2.

Subcellular localization of endogenous β-catenin in fibroblasts and cadherin transfectants, prior to and after stimulation of the Wnt/Wg signaling cascade. In the parental fibroblastic cell line, β-catenin is found in nuclei (arrow) and the cell membrane (arrowhead). In the cadherin transfectants, the β-catenin signal is restricted to the cell membrane (arrowheads). Nuclear staining of β-catenin in the cadherin transfectants (arrows) was observed only following cotransfection with β-catenin or Xwnt-8 or LiCl treatment and not after cotransfection with Xwnt-5A. Bar, 10 μm.