Fig. 2.

ZNF711 down regulation inhibits CDDP uptake and leads to CDDP resistance in EOC in vitro. (a) Immuno-blotting (IB) assays were performed to verify ZNF711 expression in the indicated stable HEY and SKOV3 cell lines. (b) An MTT cell viability assay (left) and half-maximal inhibitory concentration (IC50) value of CDDP (right) in HEY and SKOV3 cells stably expressing ZNF711 sh#1 or ZNF711 sh#2 compared to the cells expressing SH-vector control. (c) FACS analysis of Annexin V staining (left) and quantification (right) of indicated HEY and SKOV3 stable cell lines treated with vehicle or CDDP (5 μM, 24 h). (d) Representative images (left) and quantification (right) of colony number of the indicated cells treated with vehicle or CDDP (5 μM). (e) Content of intercellular CDDP in the indicated HEY and SKOV3 cells. (f) The quantification of DNA-bound CDDP (Pt) in the indicated HEY and SKOV3 cells. Each bar shown in the figure represents the mean ± SD of three independent experiments. *p < 0.05 (Kruskal-Wallis test with Bonferroni's correction).