Fig. 5.

ZNF711 regulates SLC31A1 promoter activity in EOC cells. (a) The SLC31A1 promoter luciferase reporter plasmids and Renilla pRL-TK plasmids were transfected into the indicated empty vector-transduced, ZNF711-transduced, control shRNA-transduced, and the ZNF711-shRNA transduced HEY and SKOV3 cells. After 48 h, the luciferase signal was examined and presented. (b) Schematic illustration of the P1-P7 cloned fragments of the human SLC31A1 promoter. (c and d) Luciferase activity of the SLC31A1 promoter fragments in control vector cells and ZNF711-overexpressing EOC cells. (e and f). Luciferase activity of SLC31A1 promoter fragments in shRNA control vector cells and ZNF711 knockdown EOC cells. (g) ChIP assays were performed using an anti-Flag antibody to screen three predicted ZNF711-binding sites on the SLC31A1 promoter. IgG was used as a negative control. (h) ChIP assay showing the enrichment of Pol II on region 2 in the SLC31A1 promoter in the indicated HEY and SKOV3 cells. Each bar shown in the figure represents the mean ± SD of three independent experiments. *p < 0.05 (unpaired t-test or one-way ANOVA with Bonferroni's correction).