Fig. 7.

Combined BIX-01294 sensitizes ZNF711-downregulated EOC cells to CDDP therapy. (a-b) SLC31A1 mRNA (a) and protein expression (b) in ZNF711- down regulated EOC cells treated with BIX-01294 or vehicle control. (c) MTT analysis (left) and IC50 value (right) of CDDP in the indicated cells. (d) Representative images (left) and quantification (right) of the number of colonies in the indicated cells treated with either the vehicle or BIX-01294. (e) FACS analysis of Annexin V staining (left) and quantification (right) of indicated stable HEY and SKOV3 cell lines treated with a vehicle or BIX-01294. (f) Intercellular CDDP content in the indicated cells. (g) The quantification of DNA-bound CDDP (Pt) in the indicated cells. (h) Representative images of the indicated nude mice treated with a vehicle or BIX-01294. (i) Relative change in the luminescence signals of the indicated xenograft tumors at the indicated time points. (j) Kaplan-Meier survival analysis of the indicated tumor-bearing mice. (k) Concentration of intercellular CDDP in indicated cells. (l) IHC staining of SLC31A1, PCNA, and TUNEL staining (left) and quantification (right) of SLC31A1 expression, apoptotic rate, and proliferation index in the indicated xenograft tumors. Scale bars, 50 μm. Each bar shown in the figure represents the mean ± SD of three independent experiments. *p < 0.05 (one-way ANOVA with Bonferroni's correction).