FIGURE 7.

Selenium sulfide suppresses C‐MET downstream in the AKT/mTOR and MAPK signaling pathways. (A) Protein expression of p‐AKT (S473), AKT, p‐mTOR (S2448), and mTOR in SK‐Hep‐1 and SMMC‐7721 cells treated with SeS₂ for 48 h at indicated concentrations. (B) Protein expression of p‐MEK (T286), MEK, p‐ERK (T202/Y204), and ERK in SK‐Hep‐1 and SMMC‐7721 cells treated with SeS₂ for 48 h. (C) Protein expression of p‐ERK5 (Y220), ERK5, p‐JNK (Y185), JNK, p‐p38 (T180), and p38 in SK‐Hep‐1 and SMMC‐7721 cells treated with SeS₂ for 48 h. (D) Protein expression of p‐AKT (S473), AKT, p‐MEK (T286), and MEK in SK‐Hep‐1 and SMMC‐7721 cells after treatment with 20 μM SeS₂ in the presence of 1 μM AZD5363 for 12 h or 0.5 μM AZD8330 for 6 h. E, Bar plot of (A). (F) Bar plot of (B). (G) Bar plot of (C). (H and I) Bar plot of (D). Data are expressed as the means ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group