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Design and targeting of multicistronic vectors (MCVs). CRISPR/Cas9 is designed to cut within an expression-permissive landing pad. Homology arms direct vector insertion to the landing pad by homology-directed repair. The CAG promoter is used to drive ubiquitous transgene expression (a and b) while one of several ‘endo promoters’ is used to obtain endothelial-specific expression (b). (Reprinted with permission from Eyestone W et al, 202037).
