Figure 6.

Transient RNA production is visualized by a prequenched probe.A, RNA transcript is detected by a complementary probe to RNA sequence (colored in magenta). The fluorescence signal appears when probe anneals with RNA transcript (green arrow). B, coupling with labeled protein, binding of RNAP and RNA production are visualized together to determine the timing of beginning and dissociation of transcription. RNAP entering, probe binding, and dissociation are indicated by red, green, and black arrows, respectively. The scheme is inspired by Zhang’s work (132). RNAP, RNA polymerase.