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. 2021 Aug 27;24(9):103038. doi: 10.1016/j.isci.2021.103038

Figure 1.

Figure 1

Generation of the dual fluorescent protein reporter system

(A) Schematic illustration of the pEGFP-cox8 reporter system.

(B) Schematic illustration of the pLVX-actin-mCherry reporter system.

(C) Live imaging of EGFP-Cox8-mCherry-Actin-HeLa cells within 16 hr. Arrow points to the observed dynamic changes of cell proliferation.

(D) Confocal microscopy observation of co-localization of GFP-COX8 on the mitochondria. The GFP-COX8-HeLa cells were stained with TMRE (200 nM) for 20 min before the examination by confocal microscopy. Scale bar: 10 μm.