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. 2021 Aug 27;24(9):103038. doi: 10.1016/j.isci.2021.103038

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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In situ observation of the biological effects of gamma-radiation via the dual fluorescence reporter system

(A) HeLa-cox8-EGFP-actin-mCherry cells were exposed to 1 Gy, 2 Gy, 4 Gy gamma-ray and incubated for 24 hr and observed with confocal laser scanning microscope. Green color indicates the mitochondria encoding COX8-EGFP, red color shows the actin encoded by actin-mCherry, and blue color represents the DAPI-stained nuclei. Scale bar: 5 μm.

(B) The ratio comparison for the mean fluorescence intensity (MFI) of GFP over mCherry fluorescence intensities quantified in the Figure 3A. The MFI was determined by ImageJ software. Bar graphs are presented as mean ± sd, n = 3.

(C) The cellular mean fluorescence intensity (MFI) measured by Thermo high-content imaging and screening (HCS) system. HeLa-Cox8-EGFP-actin-mCherry cells were exposed to 1 Gy, 2 Gy, and 4 Gy gamma-ray, respectively. MFI changed with time after the γ-ray irradiation.