Detection of the radiation effect on HeLa cells exposed to different radiation doses
(A) CCK-8 assay for cell viability.
(B) LDH assay for cell cytotoxicity.
(C) Colony-formation assay of HeLa cells after 14 days under different radiation doses.
(D) Flow cytometry results of Annexin V-PI assay.
(E) Apoptotic cells at different radiation doses.
(F) Cell cycle examination. The experiment was performed 3 times. Data are presented as Mean ± SD; p < 0.01(∗∗); p < 0.001(∗∗∗); ns: not significant compared with untreated cells.