Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 27;24(9):103038. doi: 10.1016/j.isci.2021.103038

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Analysis of the radiation effect on mitochondrial mass

(A) Workflow for flow cytometric analysis of mitochondrial mass.

(B) Fluorescence histogram of mitochondrial mass by flow cytometric analysis. Mitochondria of cells were labeled with Mitotracker Deep Red probes and assayed at 6, 12, and 24 hours post-radiation.

(C) Quantitative analysis of mitochondria by flow cytometry.

(D) Effect of gamma-gay irradiation on mitochondrial DNA copy number. HeLa cells were exposed to gamma-ray at doses of 1 Gy, 2 Gy and 4 Gy, respectively. After 6, 12, 24 hours, total genomic DNA was extracted and used as a template to detect the mtDNA/nDNA ratio by RT-qPCR. Data are presented as Mean ± SD; #: significant compared with unirradiated group; ∗∗p < 0.01; ∗∗∗p < 0.001.