FIG. 6.
Apparent affinity of wild-type (wt) and mutant KIX polypeptides for complex formation with fl-c-Myb (276-315) or KID (88-160) peptides, as indicated. Top, representative curve for binding of wt and Y658F mutant KIX polypeptides to each peptide. Bottom, table showing calculated Kd values for binding of wt and Y658F, K662A, K606A, Y650A, and LL652-3AA mutant KIX polypeptides. Results show means for measurements from three or more independent experiments with standard deviations. Total fluorescence intensity in each experiment remained constant with increasing concentrations of KIX protein.