Figure 2.

GITR protein turnover. WT or Traf3^−/− 2B4 cells were treated with 30 μg/ml cycloheximide (CHX) for 4 h, then stained for GITR (A) or for CD3 (B) prior to flow cytometry analysis. “Iso ctrl” indicates staining using isotype control Abs. Quantification of MFI ± SEM (n = 4) is shown to the right of a representative result in both B and C. ∗∗p < 0.01, and n.s indicates no significant difference by unpaired t test.