TABLE 2.

Quantity of chemicals used in PCR amplification

Reaction mixture	Quantity (ul)
Reaction buffer	1.0
MgCl2	0.6
Primers
(Reverse)	0.5
(Forward)	0.5
Taq DNA polymerase	0.4
dNTPs	0.2
DNA template	2.0
Water	4.8
Total	10.0