TABLE 3.
Cycle conditions (temperature and time) for PCR amplification
| Temperature (oC) | Time (mins) | No. of cycles | |
|---|---|---|---|
| Initial heating | 94 | 2 | 1 |
| Denaturation | 94 | 1 | 33 |
| Annealing | 61.5 | 1 | |
| Extension | 72 | 1.5 | |
| Final extension | 72 | 20 | 1 |
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