FIGURE 4.

Inhibition of cGAS and STING reduces tissue degeneration and cellular senescence in brain organoids. (a–e) WT and A‐T BOs where treated for one month with aspirin (4mM) or H‐151 (3 µM) starting at 5 months of development. (a) Each point in the scatter plot shows the organoid size of a single BO. At least 8 organoids per condition were analysed. ***p < 0.001; one‐way ANOVA with Tukey's multiple‐comparison post hoc corrections. (b) Representative images from quantifications shown in c. Scale bar, 0.7 mm. (c) SA‐β‐gal assays were performed on WT and A‐T BOs with the indicated treatments. Each point in the scatter plot represents a single BO section analysed. n = 3 independent experiments. *p < 0.05; ***p < 0.001; one‐way ANOVA with Tukey's multiple‐comparison post hoc corrections. (e) Total RNA from WT and A‐T BOs were used for RT‐qPCR analysis. B2M mRNA was used as normalizer. n = 3 independent biological samples. Exact P values can be found in Figure S4