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. 2021 Aug 2;40(18):e105658. doi: 10.15252/embj.2020105658

Figure EV6. Effects of host PPxPxY proteins on replication‐defective and VP30‐independent minigenome assays.

Figure EV6

  1. MG activity upon over‐expression of each of the indicated host proteins using WT or replication‐deficient MG systems. 293T cells were transfected with plasmids encoding FLAG‐tagged host proteins along with the MG plasmids. Fold MG activity was calculated relative to a no VP30 control.
  2. MG activity upon over‐expression of each of the host protein using the 5’ UTR mutant MG as a template.
  3. MG activity upon over‐expression of GFP or hnRNPUL1 peptide 3 fused to GFP in combination with full‐length (FL) hnRNPUL1 (doses 12.5 ng and 125 ng). Statistical significance was calculated relative to the GFP control for each concentration tested using ANOVA with Tukey’s multiple comparisons test.

Data information: For each panel (A–C), the data represent the mean ± SD from one representative experiment in which each transfection condition was performed in triplicate (n = 3). Each experiment was reproduced in an additional, independent experiment (see Appendix Figs S5–S7). ****P < 0.00005; ***P < 0.0005, **P < 0.005.