FIG. 4.
Incorporation into the donor plasmid is dependent upon the cross-link. (A) Neither AAAF or angelicin induce repair synthesis in the DT plasmid. After incubation in HeLa WC extract, the samples were processed and digested with NcoI and AvaII. (B) A double-strand break does not substitute for the interstrand cross-link. The CT plasmid was digested with SmaI to create a single blunt-ended double-strand break (L-CT). After incubation in the extract samples in lanes 1, 3, and 5 were digested with NcoI and AvaII. Samples in lanes 2 and 4 were digested with NcoI.