FIG. 5.

Heterologous DNAs stimulate repair synthesis to variable extents. (A) After incubation in HeLa WC extract, the samples were digested as follows: lanes 1 and 4, NcoI and BsaI; lanes 2, 3, 5, and 6, BamHI. Digestion with BamHI caused linearization of all DNAs. (B) Histogram showing quantification of results shown in panel A. Each bar represents the sum of the band intensities of the CLT plus the DT/pET28/pACYC184 minus the sum of the band intensities of the CT plus DT/pET28/pACYC184 representing the background. The absolute incorporation was then normalized by setting the value for the DT/CLT at 100%.