Table 1.
List of plasmids used for protein expression and construction of yeast strains.
| Name | Coding sequence | Purpose | Source |
|---|---|---|---|
| pSW1 | Dam1WTc with Hsk3‐6xHis | Protein purification | Westermann laboratory |
| pSW4 | Dam1‐19c with Hsk3‐6xHis (Dam1 Q205 Stop) | Protein purification | Westermann laboratory |
| pSW17 | Dam14Dc with Hsk3‐6xHis (Dam1 S20D, S257D, S265D, S292D) | Protein purification | Westermann laboratory |
| pAD21 | Dam1ΔSxIPc with Hsk3‐6xHis | Protein purification | This study |
| pTZ12 | GST‐Bim1 | Protein purification | Zimniak et al (2009) |
| pTZ15 | GST‐Bim11–133 | Protein purification | Zimniak et al (2009) |
| pTZ13 | GST‐Bim1120–344 | Protein purification | Zimniak et al (2009) |
| pTZ39 | GST‐Bim1185–344 | Protein purification | Zimniak et al (2009) |
| pTZ40 | GST‐Bim1205–344 | Protein purification | Zimniak et al (2009) |
| pET28a‐Bik1‐6xHis | Bik1‐6xHis | Protein purification | Westermann laboratory |
| Stu2‐Flag | Protein purification | Westermann laboratory | |
| StrepII‐Mps1 | Protein purification | Westermann laboratory | |
| Ndc80c‐Flag | Protein purification | Westermann laboratory | |
| pAD4 | Duo1WT‐6xFlag in pRS306 | Yeast transformation | This study |
| pAD5 | Duo1ΔSxIP‐6xFlag in pRS306 | Yeast transformation | This study |
| pDD526 | Pds1–18xMyc | Yeast transformation | Westermann laboratory |
| pAD19 | dam1–19 in pRS305 | Yeast transformation | This study |
| pAD24 | Dam1WT in pRS305 | Yeast transformation | This study |
| pSW809 | Ndc80WT in pRS306 | Yeast transformation | Lampert et al (2013) |
| pAD67 | Ndc80Δ490–510 in pRS306 | Yeast transformation | This study |
| pAD56 | Ask1WT in pRS303 | Yeast transformation | This study |
| pAD72 | Ask12A (S216A, S250A) in pRS303 | Yeast transformation | This study |
| pAD73 | Ask12D (S216D, S250D) in pRS303 | Yeast transformation | This study |