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. 2021 Aug 19;20(9):e13455. doi: 10.1111/acel.13455

FIGURE 5.

FIGURE 5

The effect of iAβo on miniature AMPAergic and evoked EPSC is PKC‐dependent. (a) mEPSC recordings of primary hippocampal neurons obtained for control (n = 11), 500 nM iAβo (n = 11), 2.5 µM chelerythrine (CLR) +500 nM iAβo (n = 12), and 2.5 µM CLR (n = 12) (V h = −60 mV). AMPA miniature currents (mEPSC) were isolated perfusing (in µM): 20 DAPV, 1 strychnine, and 10 bicuculline, in the presence of 500 nM TTX and recorded at V h = −60 mV. (b, c) mEPSC frequency (b) and amplitude quantification (c) for each of the conditions described in (a). (d) AMPA‐evoked current in the absence (n = 9) and the presence of 1000 nM iAβo (n = 9), iAβo co‐applied together with CLR (n = 11) and CLR (n = 11) (Vh = −60 mV). 100 µM AMPA was perfused to evoke maximal amplitude currents in the presence of 500 nM TTX. Segmented lines indicate maximum current reached (I MAX). (e) Average amplitude of AMPA‐evoked current for each condition in (d). Bar charts represent the average ± SEM. One‐Way Welch's ANOVA with Games–Howell post hoc test for (b): F(3, 42) = 6.11, p = 0.002. p‐values for post hoc test: control vs. iAβo: 2.27E‐2, iAβo vs. iAβo + CLR: 1.78E‐2, iAβo vs. CLR: 2.07E‐3. One‐Way Welch's ANOVA with Games–Howell post hoc test for (c): F(3, 42) = 8.54, p = 1.52E‐4. P‐values for post hoc test: control vs. iAβo: 2.65E‐4, iAβo vs. CLR: 1.23E‐2, iAβo vs. iAβo + CLR: 9.49E‐4. One‐Way Welch's ANOVA with Games–Howell post hoc test for (e): F(3, 35) = 5.88, p = 2.31E‐3. p‐values for post hoc test: control vs. iAβo: 3.31E‐3, iAβo vs. CLR: 1.10E‐2, iAβo vs. iAβo + CLR: 1.49E‐2. * denotes p < 0.05, ** p < 0.01 and *** p < 0.001