Detailed genotypes
Figure 1 .
1a‐c: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
Figure 2 .
1a‐h: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
Figure 3 .
3a: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
3b,c: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐ GAL80
3d: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
3e: y,w,ey‐FLP/ ey3.5‐Hsp70‐DmAtg13‐GFP;UAS‐RasV12/ act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
3f,g,h: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐ GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
y,w,ey‐FLP/ ey3.5‐Hsp70‐DmAtg13‐GFP;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
3i,j: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
Figure 4 .
4a: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/ act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
4b: y,w,ey‐FLP/y,w;UAS‐RasV12/ act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
4c: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/ act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
hsflp;; Frt82B, atg13Δ81/ Frt82B, atg13Δ81
hsflp;; Frt82B / Frt82B
Figure 5 .
5a: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/ act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
5b: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
5c,d: y,w,ey‐FLP/y,w;UAS‐RasV12/ act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
5e: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
5g: y,w,ey‐FLP/y,w;UAS‐RasV12/ act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
Figure EV1 .
EVF 1a: y,w,ey‐FLP/y,w;UAS‐RasV12 / act>y+ >GAL4, UAS‐GFP; Frt82B /Frt82B, tub‐GAL80
EVF 1b,c: y,w,ey‐FLP/y,w;UAS‐RasV12 / act>y+ >GAL4, UAS‐GFP; Frt82B, scrib1 /Frt82B, tub‐GAL80
EVF 1d: w1118
EVF 1e: w1118/w*, spok‐Gal4, UAS‐Dcr‐2.D; UAS‐rpr
EVF 1f:;;ecd1, st1, ca1/ecd1, st1, ca1 (Bloomington 218)
Figure EV2 .
EVF 2a: y,w,ey‐FLP/y,w;UAS‐RasV12, pmCh‐atg8a/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12, pmCh‐atg8a/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐ GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12, pmCh‐atg8a/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
EVF 2b: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+ >GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
EVF 2c: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
EVF 2d,e: y,w,ey‐FLP/y,w; g‐atg13, UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
EVF 2f: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg14Δ5.2,scrib1/Frt82B, tub‐GAL80, atg14Δ5.2
EVF 2g: y,w,ey‐FLP/y,w;UAS‐RasV12, pmCh‐atg8a/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12, pmCh‐atg8a/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐ GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12, pmCh‐atg8a/act>y+>GAL4, UAS‐GFP; Frt82B, atg14Δ5.2,scrib1/Frt82B, tub‐GAL80, atg14Δ5.2
EVF 2H: y,w;; Frt82B, atg13Δ81/Frt82B, atg13Δ81
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg14Δ5.2,scrib1/Frt82B, tub‐GAL80, atg14Δ5.2
Figure EV3 .
EVF 3a: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
EVF 3b: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
EVF 3c: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
EVF 3d: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80
y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, atg13Δ81scrib1/Frt82B, tub‐GAL80, atg13Δ81
Figure EV4 .
EVD 4a: y,w,ey‐FLP/y,w;UAS‐RasV12/act>y+>GAL4, UAS‐GFP; Frt82B, scrib1/Frt82B, tub‐GAL80