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. 2021 Aug 20;20(9):e13465. doi: 10.1111/acel.13465

FIGURE 1.

FIGURE 1

YAP is downregulated and inactivated in hippocampal astrocytes of aged mice and AD model mice. (a) Western blot analysis of p‐YAP, YAP, and Lamin B1 expression in the hippocampus of 2 M and 24 M mice. (b–d) Quantification of relative expression of p‐YAP/YAP (b), YAP (c) and Lamin B1 (d) as shown in (a) (n = 3 per group, normalized to 2 M mice). (e) Double immunostaining analysis of YAP (green) and GFAP (red) in the hippocampus of 2 M and 24 M mice. (f) Quantitative analysis of YAP+ cells over total astrocytes as shown in (e) (n = 3, normalized to 2 M mice). (g) Western blot analysis of p‐YAP, YAP, and Lamin B1 expression in the hippocampus of WT and AD model mice (6 M). (h–j) Quantification of relative expression of p‐YAP/YAP (h), YAP (i) and Lamin B1 (j) as shown in (g) (n = 3, normalized to WT mice). (k) Double immunostaining analysis of YAP (green) and GFAP (red) in the hippocampus of WT and AD model mice (6 M). (l) Quantitative analysis of YAP+ cells over total astrocytes as shown in (k) (n = 3, normalized to WT mice). Scale bar, 100 μm. Data were mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001