TABLE 1.
Type of solution | UV‐VIS absorbance data | Absorbance values of each analyte | ||||
---|---|---|---|---|---|---|
Aat 325 nm | Aat 274 nm | Aat 264 | Caffeine | Trigonelline | Chlorogenic acid | |
Aat 274–0.37A325 | Astock solution at 264 nm–Asum at 264 nm | Aat 325 | ||||
Stock solution | 0.841 | 0.457 | 0.338 | 0.395–0.37 (0.815) = 0.10975 | 0.338–0.265 = 0.0729 | 0.815 |
1st cycle extract | 0.712 | 0.342 | 0.221 | |||
2nd cycle extract | 0.103 | 0.053 | 0.0441 | |||
Sum of extracts (1stand 2ndext) | 0.815 | 0.395 | 0.2651 |