Figure EV1. De novo methyltransferases are expressed in different cell stages of adult hippocampal neurogenesis.

1. FACS strategy for isolation of neural stem and early progenitor cells (NSPCs), late progenitor cells (lPCs), and neurons from the adult mouse hippocampus. After separating single cells using their forward scatter (FSC) and side scatter (SSC) properties, dead cells were excluded based on incorporation of propidium iodide. NSPCs were isolated from Nestin::EGFP mice and lPCs from Dcx::GFP mice based on Gfp intensity. Neurons were identified based on incorporation of the neuron‐specific dye NeuroFluor (NeuO).
2. Transcript expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in NSPCs, lPCs, and neurons as determined by RT–PCR.